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KMID : 0043320100330040551
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Archives of Pharmacal Research
2010 Volume.33 No. 4 p.551 ~ p.556
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Galactosylated Chitosan-g-PEI/DNA Complexes-loaded Poly(organophosphazene) Hydrogel as a Hepatocyte Targeting Gene Delivery System
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Cho Chong-Su
Jiang Hu-Lin
Kim You-Kyoung
Lee Sun-Mi
Park Mi-Ran
Kim Eun-Mi
Jin Yong-Mei
Arote Rohidas
Jeong Hwan-Jeong
Song Soo-Chang
Cho Myung-Haing
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Abstract
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Hydrogels are widely used in drug delivery systems because they can control the release and thereby enhance the efficiency of locally delivered bioactive molecules such as therapeutic drugs, proteins, or genes. For gene delivery, localized release of plasmid DNA or polymer/DNA complexes can transfect cells and produce sustained protein production. We tested the galactosylated chitosan-graft-polyethylenimine (GC-g-PEI)/DNA complexes-loaded poly(organophosphazene) thermosensitive biodegradable hydrogel as a hepatocyte targeting gene delivery system. The poly(organophosphazene) hydrogel loaded with GC-g-PEI/DNA complexes showed low cytotoxicity and higher transfection efficiency than PEI/DNA complexes, as well as good hepatocyte specificity in vitro and in vivo. Our results indicate that poly(organophosphazene) hydrogels loaded with GC-g-PEI/DNA complexes may be a safe and efficient hepatocyte targeting gene delivery system.
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KEYWORD
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Gene therapy, Galactosylated chitosan-g-polyethylenimine, Poly(organophosphazene) hydrogel, Hepatocyte targeting, Cytotoxicity, Transfection efficiency
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